摘要
用大鼠的骨髓瘤细胞系(IR983)与经马铃薯Y病毒(PVY)免疫的大鼠(LOU/c)脾细胞融合,建立了3类分泌抗该病毒株系特异性单克隆抗体(McAB)的杂交瘤细胞系。其一对PVY^n具有特异性反虚;其二对PVY°具有特异性反应;其三对PVY^n和PVY°均产生反应。经用ELIAA双抗体夹心法和ELISA间接法检测,上述(McAb)同TMV、CMV、TEV、AMV、TuMV、PLRV、PVX七种植物病毒均不产生交叉反应。对上述杂交瘤细胞系和McAb的生物学特性及理化特性进行了鉴定。
The rat splecncytes immunized with potato virus Y (PVY) and rat myeloma (IR983) were fused by PEG(M. W. 1450). Three kinds of stable hybridoma cell lines secreting monoclonal antibodies (McAb) were derived. One kind of the cell lincs producing McAbs reacts to PVY^n specifically. Another reacts to PV^o specifically. The third cne reacts to both of the two strains. Tested by the methods of sandwich-ELISA and indirect-ELISA, all kinds of McAbs did not react to seven plant viruses: tobacco mosaic (TMV), cucumber mosaic (CMV), tobacco tech (TEV), alfalfa mosaic (AMV), turnip mosaic (TuMV), potato leaf roll (PLRV), and potato virus X (PVX). The properties of the hybridoma cell lines and the McAbs were tested.
出处
《生物工程学报》
CAS
CSCD
北大核心
1990年第3期230-235,共6页
Chinese Journal of Biotechnology
基金
国家科委生物工程开发中心资助
