摘要
用大豆花叶病毒免疫的BALB/c小鼠脾细胞与Sq2/0鼠骨髓瘤细胞融合,获得3株分泌抗大豆花叶病毒单克隆抗体的杂交瘤细胞株,S—1、S—2和S—3。它们的鼠腹水滴度高达1:10~7,能被大豆花叶病毒兔抗血清所阻断。单克隆抗体与其它三种植物病毒不起反应,对大豆花叶病毒的不同株系有特异性差异。
Three monoclonal antibodies against SMV, namely S-1, S-2 and S-3, were obtained by fusion of Sp2/o myeloma cells with spleen cells from BAL-B/c mice immunized with SMV. The titers of their ascite fluid were all more than 1:10~6 by the indirect ELISA.The ability of three McAbs could be blocked by rabbit antiserum against SMV.In the immunosorbent electron microscopy, McAbs reacted with SMV, but not with cowpea aphid-borne mosaic, watermelon mosaic and tobacco mosaic viruses. S-1, S-2 and S-3 were specific to strains of SMV,
出处
《植物病理学报》
CAS
CSCD
北大核心
1990年第2期131-133,共3页
Acta Phytopathologica Sinica
