摘要
采用静态方法从多种大孔树脂中筛选出Ｘ＿５，ＡＢ＿８两种树脂，它们对紫草色素具有较好的吸附能力，同时对糖吸附弱，多次高温灭菌后进行的吸附实验显示其吸附性能变化不大．摇瓶培养实验结果表明：树脂的最佳添加量为６．７％，色素的产量比常规培养分别增加了５．２和６倍．反应器与ＡＢ＿８树脂柱相偶联进行同时分离色素的培养实验，结果使色素产量增加了５．３倍．
Two kinds resin(X_5, AB_8) were chosen from six kinds of resin. They adsorb more Shikonin and less sugar. They can be used repeatedly and sterilized many times under the temperature 121 ℃, and their adsorbent properities change little. In flask culture, when the optimum quantity of resins was 6.7%, the Shikonin production increased by 5.2 and 6 fold respectively. An air_lift bioreactor system equipped with a AB_8 column was established, which stimulated Shikonin production by 5.3 fold.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1999年第6期27-31,共5页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省自然科学基金
关键词
深层培养
紫草色素
细胞培养
吸附树脂
Lithospermum erythrorhizon
submerged culture
Shikonin
separation
adsorbent
