摘要
在 1 2MS液体培养基上研究了不同理化因子对水母雪莲毛状根生长和总黄酮生物合成的影响。实验结果表明 :氮源总浓度 (包括NH+4和NO-3)为 30mmol L ;NH+4 NO-3比例为 5∶2 5 ;2 %蔗糖和 3%葡萄糖组合 ;0 . 5mg LGA3和 0 . 5mg LIBA ;pH5 8;18h d的光照 (光强为 35 .0 0lx) ;2 4℃ ;摇床转速为 10 0r min有利于毛状根生长及总黄酮的生物合成。在此培养条件下 ,经过2 1d的培养毛状根生长量达到 12. 8g L(DW) ,总黄酮合成量为 192 .2mg L ,即总黄酮含量占毛状根干重的 15 % ,约为干重野生水母雪莲植株总黄酮含量的 2 5倍。
The effects of different physical and chemical factors on hairy root g rowth and flavonoids production were studied in suspension culture of Saussure a medusa hairy root in 1/2 MS medium. The results showed that the following cu ltur e conditions,nitrogen concentratiaon(involved NH+ 4 and NO- 3),30mmol/L;th e ratio of ammonium to nitrate,5:25;the combination of 2% sucrose and 3% glucose; 0.5mg / L GA 3; 0.5mg/L IBA; initial pH 5.8;light cycle,18h/d(3500lx);temperature,2 4℃ ; shaker revolutions per minute,100r/min,were favourable to hairy root growth an d flavonoids production.Under the above culture conditions,up to 12.8g/L(DW) o f hairy root and 1922mg/L of flavonoids were obtained after 21 days of culture.T he content of total flavonoids in hairy root was 15%,which was about 25 times as that in the wild plantlet.
出处
《生物工程学报》
CAS
CSCD
北大核心
2005年第2期233-238,共6页
Chinese Journal of Biotechnology
基金
国家自然科学基金资助项目 (No .3 0 472 15 8)~~
