摘要
从化纤厂土样中分离得到菌株AC-4,碱性纤维素酶活力达0.602 IU/mL。通过分析菌株形态学特性、培养特征及16S rDNA序列,确定该菌株为短小芽孢杆菌。对该菌所产碱性纤维素酶的酶学性质进行测定,确定其最适作用pH为9.5,最适作用温度为50℃。
A strain producing alkaline cellulase was isolated from the soil collected from chemical fiber factory.The alkaline cellulase activity reach 0.602 IU/mL.Based on its morphologic and cultural characteristics and the sequence of 16S rDNA,the strain was determined as Bacillus pumilus.The optimum reaction pH of the alkaline cellulase is 9.5,and the optimum reaction temperature is 50℃.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第9期205-209,共5页
Biotechnology Bulletin
基金
国家基础科学人才培养基金项目(J0730648)
