摘要
利用超滤技术分离大豆多肽酶解物,得到分子量在5 KDa～10 KDa与小于5 KDa的多肽,利用高速逆流色谱技术(HSCCC)对分子量小于5 KDa的多肽进行分离,并利用反相高效液相色谱技术(RP-HLPC)进一步纯化,得到纯度为73.68%的大豆多肽。与未纯化的大豆蛋白酶解物相比,纯化后的大豆多肽清除超氧阴离子自由基和过氧化氢的能力分别提高了43.72%和71.64%。因此,该系列纯化方法所得产物的纯度高,抗氧化能力强,适合于抗氧化大豆多肽的制备。
The experiment isolated the soybean protein enzymolysis products by the ultra-filtration technique and obtained two separated components,the peptides between 10 KDa and 5 KDa and less than 5 KDa.The peptides of less than 5 KDa were separated by high-speed counter-current chromatography（HSCCC） and then further purified by reversed phase-high performance liquid chromatography（RP-HLPC）.At last get purified soybean peptides which purity was 73.68%.Compared with the soybean protein enzymolysis products,the ability of purified soybean peptides to scavenge superoxide anion radical and hydrogen peroxide had increased by 43.72% and 71.64%,respectively.Results suggest the adopted method is suitable for the preparation of soybean peptides,considering the purified products are high in purity and antioxidant ability.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第4期677-680,687,共5页
Soybean Science
基金
上海海洋大学骆肇荛大学生科技创新基金资助项目(G-7101-00-000737)
关键词
抗氧化
大豆多肽
纯化
Anti-oxidant
Soybean peptides
Purification
