摘要
组织工程支架材料是构建组织工程器官或组织的重要基础,天然脱细胞基质由于具有其优良特性而成为理想的支架材料。本研究采用胰酶去污剂法、冻融去污剂24 h法、冻融去污剂48 h法、冻融核酸酶法和去污剂核酸酶法等5种方法对牛心包组织进行脱细胞处理。通过HE染色、扫描电镜观察、含水量检测、力学检测、DNA含量检测和细胞毒性试验,判断不同脱细胞方法的脱细胞效果及其对牛心包基质的影响,从而筛选出对牛心包进行脱细胞的最佳方法。结果显示冻融后去污剂24 h法可以完全脱除牛心包的细胞成分,同时细胞外基质和力学特性保持完好,细胞毒性低,且经济实惠,是制备牛心包脱细胞支架的较好方法。
The scaffold material is critical in building a tissue-engineered organ or tissue.Extracellular matrix has become an ideal scaffold material because of its fine features.In this study,the bovine pericardiums were treated with 5 methods,including trypsin-detergent method,freeze-thaw-detergent 24 h method,freeze-thaw-detergent 48 h method,freeze-thaw-nuclease method and detergent-nuclease method.The effects of decellularization and the changes in physical properties of the matrix were tested by HE staining,scanning electron microscope,water content testing,mechanical testing,DNA content detecting and cytotoxicity.The results showed that Freeze-thaw-detergent 24 h method could remove the cell components of bovine pericardium effectively,while maintaining the major structural components and the histological and biological properties of bovine pericardium,and with low cytotoxicity.The freeze-thaw-detergent 24 h method could be the best choice to produce a decellularized bovine pericardium.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2010年第4期607-613,共7页
Chinese Journal of Biomedical Engineering
基金
国家高技术研究发展(863)计划(2007AA02Z444)
关键词
牛心包
脱细胞
冻融法
支架
去垢剂
bovine pericardium
decellularization
freeze-thaw method
scaffold
detergent
