摘要
目的:用不同方法去除猪胸主动脉壁细胞,并比较去细胞效果,为组织工程瓣膜或带瓣管道提供良好的实验材料。方法:采用十二烷基硫酸钠、胆脂酸钠、曲拉通(TritonX100)制备猪胸主动脉脱细胞基质。将实验分为十二烷基硫酸钠组、胆脂酸钠、曲拉通组和空白对照组。标本进行大体、光镜、电镜观察,力学性能的检测并做比较。结果:胆脂酸钠无法完全脱除主动脉壁内细胞,十二烷基硫酸钠和曲拉通都能完全脱除猪胸主动脉细胞,曲拉通较好地保持了胶原纤维和弹性纤维的原有排列和分布,并有较好的力学性能。后两者脱细胞时间无显著差别。结论:曲拉通可以成功用于制备大血管脱细胞基质,为组织工程瓣膜或带瓣管道的研究提供材料。
AIM: To investigate methods to remove cellular compenents from porcine thoracic aortas,resulting scaffolds of acellular tissue matrix(ACTM) for tissue engneering of heart valve or vessel. METHODS:Different detergents (Triton X-100,Sodium dodecyl sulfate and sodium deoxycholate)and enzyme (trypsin) extraction was practiced in this study. According to the different detergents, specimens of porcine thoracic aortas devided into three groups(Sodium dodecyl sulfate,sodium deoxycholate,Triton X-100). Specimens were observed grossly and the changes of mechanical properties were also studied.Haematoxylin-eosin a was performed to confirm the removal of cells. Transmission electron microscope was used to observe the integrity of collagen and elastin. RESULTS:The cells were removed effectively from porcine thoracic aortas by Triton X-100 and sodium dodecyl sulfate, and histological analysis showed that the major structural compenents of porcine thoracic aortas decelluarized by Triton X-100 had been maintained.While sodium deoxycholate were failed for porcine thoracic aortas decellularization. the collagen and elastin were kept intact. CONCLUSION:ACTM of vessel can be obtained successfully through this procedure by Triton X-100.
出处
《心脏杂志》
CAS
2005年第3期207-209,共3页
Chinese Heart Journal
基金
国家自然科学基金资助项目:(编号30271292)
