摘要
目的探讨新型生物交联剂京尼平对脱细胞猪膀胱性能的影响,为女性盆底功能重建提供理想的组织工程补片。方法采用表面活性剂+酶消化法对猪膀胱进行脱细胞处理后,用0.625%京尼平溶液交联脱细胞膀胱72 h。通过HE、Weigert弹力纤维+VanGieson结缔组织(ET+VG)染色及扫描电镜进行组织形态观察,使用万能材料试验机检测材料生物力学性能,并对交联前后的脱细胞猪膀胱进行厚度、吸水性、体外胶原酶降解性能测定,以L929细胞为实验细胞,采用MTT法评估材料的细胞毒性。结果京尼平交联脱细胞猪膀胱后呈深蓝色,保持了天然组织构架的完整,胶原纤维更加致密,吸水性提高,力学性能良好,脱细胞猪膀胱在第7天已完全降解,交联后在第28天降解率为2%。交联前后材料的细胞毒性为0～1级。结论经天然交联剂京尼平交联的脱细胞猪膀胱吸水性好,具有良好的生物力学性能,抗酶解能力强且无细胞毒性,是一种较为理想的盆底重建材料。
investigate the characteristics and cytocompatibility of acellular porcine bladder fixed with genipin in vitro in order to provide ideal biomaterials for the reconstruction of female pelvis. Methods Cellular components of porcine bladder were removed by detergent-enzymatic method. The acellular porcine bladder was erosslinked with 0. 625% genipin for 72 h. The morphology of the obtained bladder was examined with hematoxylin and eosin staining, elastin/van Gieson staining and observed with scanning electron microscopy. Properties of the crosslinked acellular matrix were evaluated for tissue thickness, water absorption and in vitro enzymatic degradation. The cytotoxieity of the genipin-fixed materials was evaluated in vitro by MTT assay using a murine fibroblast cell line L-929. Results The genipin-crosslinked acellular porcine bladder was dark blue, with very well preserved collagen fibers. Cosslinked tissues had strong hydrophilicity and good mechanical properties, with a degradation ratio of only 2% after collagenase degradation for 28 d. While, the acellular porcine bladder was degradated completely at 7 d after collagenase treatment. The cytotoxicity of the materials was scored as grade 0 to 1. Conclusion The genipin-fixed aeellular porcine bladder possesses the ability of good hydrophilia, cytocompatibility and resistance to enzymatic degradation. It can be used as an ideal tissue engineering material for the reconstruction of female pelvis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第19期1985-1988,共4页
Journal of Third Military Medical University
关键词
京尼平
脱细胞猪膀胱
交联
力学性能
细胞毒性
genipin
acellular porcine bladder
crosslink
mechanical properties
cytotoxicityCorresponding author： Xu Huicheng, E-mail： xhuicheng@ yahoo, com. cn
