摘要
本实验以大麦叶肉原生质体为材料,采用常规原生质体分离培养方法,在含有1.5mg/l2,4-D、0.5 mg/l 6-BA和0.2 mg/l IAA的D_(2α)培养基中诱导了原生质体持续分裂,形成了小愈伤组织,但它未能进一步发育分化产生绿苗。通过对原生质体分离、分裂影响因素的探讨,我们认为材料的生理状态以及遗传背景在培养中起了关键作用。
Large number of mesophyll protoplasts were isolated from barley leaves, subsequently sustained cell division was induced and microcalli formed when cultured on D_2a medium supplemented with 1.5mg/l 2,4-D, 0.5mg/1 6-BA and 0.2mg/1 IAA. But the microcalli could not develop further. After many attempts, we suggest that the critical factors seem to be associated with physiological state and genetic background of materials.
出处
《上海农业学报》
CSCD
1990年第4期17-20,共4页
Acta Agriculturae Shanghai
基金
上海市科学技术委员会自然科学基金
关键词
大麦
叶肉原生质体
分离
培养
细胞
Cereal, Barley, Mesopyll protoplast, Cell division
