摘要
试验结果表明:3 ppm S-3307浸种40min可以提高叶肉原生质体的稳定性。培养方法以琼脂糖小块培养法最好,液体浅层培养法次之,看护培养又次之。原生质体的接种密度以0.5～1.0X 10<sup>6</sup>个/ml为好。原生质体进入培养后宜当天即加液。基本培养基KPR优于D<sub>2</sub>。培养基中添加0.25m tool/L腐胺或lmg/L亚精胺均不利于原生质体的细胞分裂。添加3mg/L IAA可促进细胞分裂,但不能延缓后期细胞死亡进程。添加250mg/L CH略可促进细胞分裂,延缓后期细胞死亡进程。添加1％BSA和lm mol谷胱甘肽可促进细胞分裂并能延缓细胞死亡2～3d,但异常分裂增多。
According to the results of this study, soaking barley seeds with 3ppm S3307 for 40 minutes can improve the stability of the mesophyll protoplasts. Agarose block method is the best culture method, thin layer liquid culture method the second, and nurse culture method the third. The suitable protoplast density in the mesophyll protoplast culture is 0.5～1.0×10~6 protoplasts/ml. It is suitable to add liquid medium on the first day of culture. In comparison with D_2 medium, KPR medium is better than D_2 medium.Supplementation with 0.25m mol/L putrescine or 1 mg/L spermidine can decrease the M.I. of protoplasts. Supplementation with 3 mg/L IAA can improve cell division, but can not retard the progress of cell senescence and death. Supplementation with 250 mg/L CH can improve cell division and retard the progress of cell death. Supplementation with 1％ BSA and 1 m mol/L glutathione can improve cell division and retard the progress of cell death for2～3 days, but it can also lead to the increase of abnormal division.
出处
《上海农业学报》
CSCD
1993年第3期15-21,共7页
Acta Agriculturae Shanghai
基金
浙江省自然科学基金
关键词
大麦
叶肉
原生质体
培养方法
Barley mesophyll protoplast
S-3307
Culture method
Polyamine
IAA
BSA
