摘要
在减数第一次分裂的早前期,把黑麦花粉母细胞以花粉母细胞丝状体的形式分离出来,培养在微量培养室内直接进行了观察。在这种可以使花粉母细胞保持完整并处于生活状态的条件下,仍看到有细胞融合现象存在,细胞融合的特征也与以前使用压片和切片法所看到的基本相同。在花粉母细胞丝状体液体悬浮培养条件下,花粉母细胞能进一步发育,并有部分花粉母细胞减数分裂行为表现异常,主要包括染色质的量和染色体的数目增加和减少。本研究进一步证实了我们早期所作的机械损伤和固定液的作用不是细胞融合的原因和细胞融合可导致遗传物质基础发生变化的结论。
The microsporocytes, being in early prophase of meiosis I, of rye(Secale cereal L.) were isolated out of their anther sacs as a filament,cultured in a microchamber and observated directly under the micros-cope. In this condition that the microsporocytes kept intact and livingstate, cytomixis activity still existed and showed the same ways asthat seen in the preparations of squashing and section did. Under thecondition of the liquid suspension culture of microsporocyte filament,the microsporocytes were able to continue developing and part of microsporocytes became abnormal in the meiotic behavior. The abnor-mal behavior mainly included an increase and a decrese of chromatinand chromosome number. This study futher proved our early conclu-sions that the action of machnical injuries and fixatives is not the cau-se of cytomixis and that cytomixis might induce the genome of somecells to change.
出处
《西北植物学报》
CAS
CSCD
北大核心
1991年第4期261-265,共5页
Acta Botanica Boreali-Occidentalia Sinica
关键词
黑麦
花粉母细胞
细胞融合
Microsporocyte filament
Microchamber culture
Cytomixis
