摘要
目的和方法：研究汉防己碱（Ｔｅｔ）对缺血状态下大鼠皮层神经元膜电压依赖性钾通道变化的影响。方法：分离大鼠皮层神经元和建立细胞贴附膜片钳技术。结果：在缺血状态下，钾通道开放时间常数τ１和τ２分别由对照组的０４２９ｍｓ和８２０９ｍｓ增加至１８５５ｍｓ和１７４６４ｍｓ（Ｐ＜００１），开放概率由０１１４增加到０１５１（Ｐ＜００１）。Ｔｅｔ（７５，１５和３０μｍｏｌ／Ｌ）浓度依赖性抑制由缺血所诱导的钾通道开放。Ｔｅｔ７５和１５μｍｏｌ／Ｌ改变了通道的关闭模式，３０μｍｏｌ／Ｌ改变了通道的开放模式。结论：缺血可导致神经元电压依赖性钾通道开放显著增加，对胞外钾离子聚积起重要作用，从而参与神经元的损伤。
AIM: To study the effects of tetrandrine(Tet) on
changes of rat cortical neuronal potassium channel in ischemia-like condition.
METHODS:Acutely isolated rat cortical neurons and single channel recording of patch clamp
technique. RESULTS:Compared with control group, the open time constants τ 1 and τ 2 of
potassium channels of rat cortical neurons increased from 0.429 ms and 8.209 ms to 1.855 ms
and 17.464 ms, respectively ( P all <0.01). Open-state probability increased from 0.114 to
0.151( P <0 01) Tet (7.5, 15 and 30 μmol/L) significantly inhibited the open of potassium
channel induced by ischemia-like condition in concentration-dependent maner. Tet in 30 μ
mol/L changed the open model,15 and 7.5 μmol/L changed the close model. CONCLUSION:The
prolonged open of potassium channels in ischemia-like condition would result in extracellular K
+ assembling, which play an important role in cerebral injury. Tet protected the cerebral injury
by inhibiting the long-lasting open of potassium channel induced by ischemia-like condition.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第3期260-263,共4页
Chinese Journal of Pathophysiology
