摘要
采用微电极技术对分散培养的新生大鼠海马神经元进行细胞内记录，对其被动膜电性质、Ⅰ－Ｖ关系曲线、动作电位等进行了观察。测得静息电位为－６４±１１ｍＶ（ｎ＝９６），膜阻抗为８０±２０ＭΩ，时间常数５．６±１．４ｍｓ，动作电位幅度６９±７ｍＶ（ｎ＝１５），阈电位－４８±８ｍＶ，超射值７±３ｍＶ。利用压力脉冲微量给药技术将乙酰胆碱或谷氨酸钠施加于所观察的细胞表面，发现二者均引起神经元去极化，并伴有强烈的放电反应。证明新生大鼠海马培养神经元细胞内记录是一种稳定可靠的电生理学研究方法。
With the microelectode technique intracellular recordings were taken from the neonatal rat hippocampal neurons in culture. The results showed that the input resistance(80 ±20MΩ),rest-ing membrane potential(-64 ±11 mV),membrane time constant(5.6±1.4 ms),membranecapacitance(69±17pF),spike size(69 ± 7mV),threshold level(-48 ± 8mV)and overshoot of theaction potential(7±3 mV) could be recorded from cultured hippocampal cells of neonatal rats.These electrophysiological parameters were similar to those observed in previous studies.Pressureapplication of acetylcholine(0.1mmol/L)and glutamate(0.1 mmol/L)caused depolarizationaccompanied by action potential discharge.The results suggest that the cultured neurons possessstable electrophysiological properties and the intracellular recording techniq ue for culturedhippocampal neurons is useful in the study of electrophysiology.
出处
《中国应用生理学杂志》
CSCD
1994年第2期183-185,共3页
Chinese Journal of Applied Physiology
关键词
海马
细胞培养
细胞内记录
电生理
hippocampus
cell culture
intracellular recording
membrane potential
acetylcholine
glutamate
