摘要
目的：研究“缺血”大鼠大脑大皮层神经元Ｎ－甲基－Ｄ－天冬氨酸（ＮＭＤＡ）受体通道的单通道变化特征。方法：细胞贴附式膜片钳技术。结果：在“缺血”状态下，ＮＭＤＡ受体通道３５ｐＳ和１００ｐＳ电导水平的开放概率分别由对照组的０．０７９±０．００６和０．０６７±０．００４增加到０．３０８±０．１５５和０．４８８±０．１２６（Ｐ＜０．０１），３５ｐＳ通道开放时间常数τ２由对照值（４．１７±０．３８）ｍｓ增加到（８．５４±２．０５）ｍｓ（Ｐ＜０．０１），关闭时间由（７５．５０±１４．１０）ｍｓ缩短到（１１．８０±４．３０）ｍｓ（Ｐ＜０．０１）。结论：“缺血”可显著开放大鼠大脑皮层神经元ＮＭＤＡ受体通道，使钙内流增加，对胞内钙超载起重要作用，此可能是脑缺血细胞损伤的机制之一。
AIM: To study the effects of “ischemia” on N-methyl-D-aspartate (NMDA) receptor-channels in isolated rat cerebral cortical neurons.METHODS:Cell-attached configuration of patch clamp technique.RESULTS:During “ischemia”, the open probability of 35 pS and 100 pS NMDA-receptor channels increased from control values 0.079±0.006 and 0.067±0.004 to 0.308±0.155 and 0.488±0.126, respectively( P <0 01). The open time constant τ 2 of 35 pS channels increased from (4.17±0.38) ms to (8.54±2.05) ms( P <0 01) The close time constant τ decreased from (75.50±14.10) ms to (11.80±4.30) ms ( P <0 01).CONCLUSION:NMDA-receptor-channels in rat cerebral cortical neurons were activated significantly during “ischemia”, which increases Ca 2+ inflow and plays and important role in intracellular Ca 2+ overload. It was one of the cell injury mechanisms during cerebral ischemia.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第7期613-615,共3页
Chinese Journal of Pathophysiology
基金
第三军医大学科研基金
关键词
神经元
脑缺血
受体
离子通道
NMDA
Ischemia
Neurons
Receptors, N-methyl-D-aspartate
Ion channels
