摘要
目的:观察胃癌患者血管内皮生长因子C和D(vascular endothelial growth factor-Cand-D,VEGF-C,VEGF-D)在胃癌组织中的表达以及其与肿瘤微血管生成的关系。方法:采用免疫组化S-P法检测30例胃癌组织和30例正常胃组织中VEGF-C和VEGF-D蛋白的表达,检测微血管密度(microvessel density,MVD)。结果:胃癌组织VEGF-C和VEGF-D表达明显高于正常胃组织(p<0.01),其中淋巴结转移组VEGF-C、VEGF-D的表达与淋巴结未转移组间差异显著(p<0.05)。胃癌组织MVD值明显高于正常胃组织(p<0.01),淋巴结转移组MVD值明显高于未转移组(p<0.01)。MVD与VEGF-C的蛋白在胃癌组织中的表达高度相关(r=0.735,p<0.05),与VEGF-D的蛋白在胃癌组织中的表达成正相关(r=0.623,p<0.05)。结论:VEGF-C和VEGF-D的高表达与肿瘤微血管生成及淋巴道转移密切相关,可作为评估胃癌患者预后的重要参考指标。
Objective：To explore the expression of vascular endothelial growth factor C and D in gastric cancer and its relationship with tumor angiogenesis and lymph node metastasis.Methods：Immunohistochemistry（SP） was used to detect the expression of VEGF-C and VEGF-D protein in 30 samples of gastric cancer tissue and 30 cases of normal gastric tissue.The microvessel density（MVD） was calculated.Results：The VEGF-C and VEGF-D positive expression rate in gastric cancer tissue was significantly higher than normal gastric tissues（p0.01）.In gastric cancer group,the VEGF-C and VEGF-D expression of patients with lymph node metastasis group were significantly different from those without（p0.05）.MVD in gastric carcinoma group was significantly higher than the normal gastric tissues group（p0.01）,and the MVD of lymph node metastasis group was significantly higher than that of the un-transfer group（p0.01）.MVD was highly correlated with VEGF-C of the protein in gastric carcinoma（r = 0.735,p0.05）,and positively correlated with VEGF-D of the protein in gastric cancer tissues（r = 0.623,p0.05）.Conclusion：The high expression of VEGF-C and VEGF-D in gastric cancer is closely related with tumor angiogenesis and lymph node metastasis.It can serve as the important reference indicator to assess the prognosis of gastric cancer patients.
出处
《现代生物医学进展》
CAS
2010年第12期2316-2318,共3页
Progress in Modern Biomedicine
关键词
胃癌
VEGF-C
VEGF-D
淋巴结转移
MVD
Gastric cancer
Vascular endothelial growth factor-C
Vascular endothelial growth factor-D
Lymph node metastases
Microvessel density
