摘要
目的 观察去甲二氢愈创木酸 (NDGA)对人恶性胶质瘤细胞系SHG 4 4细胞周期的影响。方法 采用细胞培养、免疫组化、细胞计数及流式细胞仪检测等技术方法。结果 ①在 2 0 0 μM浓度范围内 ,NDGA对SHG 4 4细胞增殖有抑制作用 ,使细胞增殖受阻于G1→S期 ,且这种作用与浓度呈正相关 ;②在NDGA处理 96小时内 ,NDGA对SHG 4 4细胞的增殖抑制作用以处理后 2 4小时最明显 ;③NDGA处理后 ,SHG 4 4细胞cyclinD1和CDK4 蛋白表达减弱 ,p16蛋白表达增强。结论 NDGA对SHG 4 4细胞周期有明显的增殖抑制作用 ,且这种作用可能与CDK4 蛋白激酶活性降低有关。
Objective To observe effects of nordihydroguaiaretic acid(NDGA) on the cell cycle of human glioma cell line SHG 44 cells.Methods The techniques of cell culture,immunohistochemisty,cell counts and FCM were employed in this study.Results ①A concentration dependent proliferation inhibition was demonstrated in the SHG 44 cells incubated for 24 hours in the presence of NDGA,and cell proliferation was blocked in the Gl→S phase.②The most conspicuous inhibition was measured at 24 hours after treatment with 100μM NDGA for up to 96 hours.③The expression of CDK4 and cyclin Dl protein downregulated,and P16 upregulated in the cells after NDGA treatment.Conclusion NDGA can inhibit proliferation of human malignant glioma cells,and the proliferation inhibition is correlated with the decreased activity of CDK4.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2000年第1期1-3,共3页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金! (NO 3 9670 2 96)
军队医药卫生科研基金! ( 96M0 89)
关键词
胶质瘤
脑肿瘤
NDGA
细胞周期
Nordihydroguaiaretic acid(NDGA)
Glioma
Induced differentiation
