摘要
目的筛选ＳＩＥＡ抗卵胚胎发育的候选分子。方法小鼠再感染日本血吸虫尾蚴用ＳＩＥＡ免疫后４９天，实验组收集该免疫血清培养日本血吸虫未成熟卵。对照组用未免疫小鼠相同天数感染的血清培养，２天后，收集虫卵，作免疫沉淀和ＳＤＳ—ＰＡＧＥ分析。结果发现实验组仅出现５４ＫＤ。结论日本血吸虫未成熟卵可溶性抗原中５４ＫＤ分子可能位于未成熟卵膜或基质中，由未成熟卵所释放。
Objective The study aims on sifting the candidate molecule of anti embryonation.Methods The immature eggs were cultivaed with sera from mice immuned by Schistosome japonicum soluble immature egg (SIEA) and infected later or normal infected sera respectively.Two days later,the eggs were collected,immune preciapated,and analysed by SDS PAGE.Results 54KD was the only common band in sample from the eggs cultivated by infected sera after immunity;no band in eggs cultivated by infected sera was found.Conclusion The resluts indicated that the immunity elicited by 54KD of SIEA may play a important role in anti embryonation.
出处
《中国地方病学杂志》
CSCD
1999年第1期7-9,共3页
Chinese Jouranl of Endemiology
基金
湖北省自然科学基金
