摘要
本文建立了快速高分离度液相色谱-串联质谱(RRLC-MSMS)测定番茄红素的方法,通过实验优化了的分析条件。选择m/z109为定量离子,碰撞能量20V;m/z413为定性离子,碰撞能量10V。在0.02～10.0mg/L浓度范围内,曲线方程:Y=718.79x2+5964.98x-111.64,R2=0.999998。S/N=3为定性限,检出浓度0.03mg/L;S/N=10为定量限,检出浓度0.1mg/L。用1.0mg/L的溶液连续进样6次,结果的精密度RSD=3.97%。测定方法中样品前处理简单,节省溶剂,用时短,定性、定量准确,满足检测分析的要求。
A method was developed for the determination of lycopene by rapid resolution liquid chromatography-tandem mass spectrometry （RRLC-MSMS） and the analytical method was optimized by the experiment.Quantitative ion was m/z109,collision energy was 20V,qualitative ion was m/z413 and collision energy was 10V.The concentration range was from 0.02mg/L to 10.0mg/L and curvilinear equation was Y=718.79x^2＋5964.98x-111.64,R^2=0.999998.S/N=3 was qualitative limit and concentration of detection was 0.03mg/L.S/N=10 was quantitative limit,detection concentration was 0.1mg/L,the solution which was 1.0mg/L was injected 6 times continuously,RSD of the precision was 3.97%.
出处
《中国食物与营养》
2010年第6期54-56,共3页
Food and Nutrition in China
