摘要
建立保健食品中番茄红素的液相色谱-串联质谱测定方法。样品经氢氧化钾溶液乙醇皂化,去除脂肪后用质量浓度为1g/100mL的2,6-二叔丁基-4-甲基苯酚(BHT)石油醚溶液提取,提取液经中性氧化铝固相萃取柱净化,采用液相色谱-质谱联用技术-大气压化学电离源多反应监测模式检测,外标法定量。方法的检出限和定量限分别为10μg/kg和100μg/kg,在50～5000μg/kg添加水平范围内,平均回收率为88.0%～103.1%,相对标准偏差低于15%。本方法灵敏,为保健食品中番茄红素的定量检测和确证提供了新的手段。
A comprehensive analytical method based on liquid chromatography-tandem mass spectrometry（LC-MS/ MS） was developed for the determination of lycopene in dietary supplements.Samples were saponified to remove lipid,followed by vortex-assisted extraction with petroleum ether containing 1 g/100 mL 2,6-di-tert-butyl-4-methylphenol（BHT）.The resulting extract was further cleaned up on a neutral alumina solid-phase extraction cartridge.The pooled eluate was determined on LC-MS/MS by atmospheric pressure chemical ionization coupled with a multiple reaction-monitoring（MRM） mode.Lycopene was quantified by external standard method.The limit of detection（LOD） was 10 μg/kg,and the limit of quantification（LOQ） was 100 μg/kg.The average recovery rates of lycopene across six spike levels,50.0,100.0,250.0,500.0,2500.0 μg/kg and 5000.0 μg/kg were 88.0%–103.1% with a relative standard deviation less than 15%（n=6）.This method is sensitive and provides a new approach for the quantitative analysis and validation of lycopene in dietary supplements.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第20期206-211,共6页
Food Science
基金
“十一五”国家科技科技支撑计划项目(2006BAD27B02)
国家质检总局科研项目(2007IK188)
重庆市科委科研项目(CSTC2009CB1012)
关键词
番茄红素
保健食品
测定
液相色谱-串联质谱法
lycopene
dietary supplements
determination
liquid chromatography-tandem mass spectrometry
