摘要
采用HPLC法研究了番茄油树脂中番茄红素的测定方法 .结果表明最佳的色谱分离条件为流动相采用V(乙腈 ) :V(甲醇 ) :V(二氯甲烷 ) =7∶7∶2的体系、检测波长 4 72nm ,在此条件下可以很好地分离番茄红素、β 胡萝卜素及其异构体 ,番茄红素和 β 胡萝卜素的保留时间分别为 9～10min和 13～ 14min .保留时间和样品的重复性试验表明 ,变异系数均低于 5 % ,回收率范围在98%～ 10
The content of lycopene in tomato oleoresin was determined by HPLC. The results showed that the best mobile phase was acetonitrile: methanol: dichloromethane (7∶7∶2) detected at 472nm. The retention times of lycopene and beta carotene were 9～10 minutes and 13～14 minutes respectively. The reproductive SD of lycopene was lower than 5 percent and the recovery rate was 98～101 percent.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2001年第5期506-509,共4页
Journal of Wuxi University of Light Industry
