摘要
通过外植体类型、生长素和细胞分裂素浓度等因素对紫花苜蓿愈伤组织诱导、分化影响的试验,建立了高效的紫花苜蓿组织培养体系,即以真叶为外植体,在MS+0.2mg/L2,4-D+0.01mg/LNAA+0.2mg/LKT培养基上进行愈伤组织的诱导,诱导率可达到100%,愈伤组织继代2～3次,然后在MS培养基上分化,分化率可达90%。分化苗转入1/2MS培养基进行生根,形成完整的组培苗。愈伤组织诱导的正交试验表明,2,4-D浓度是影响苜蓿愈伤组织诱导率的主要因素,其次是外植体类型、NAA浓度、6-BA浓度和KT浓度;对苜蓿愈伤褐化率影响较大的因素是外植体类型,其次是KT、2,4-D、6-BA、NAA浓度。外植体类型对愈伤组织诱导率和褐化率的影响均达到极显著水平(P<0.01);生长素(2,4-D、NAA)对苜蓿愈伤组织的诱导作用大于细胞分裂素(6-BA、KT)。分化试验表明,紫花苜蓿愈伤组织分化的主要决定因素是愈伤诱导培养基的激素配比。
The high efficiency alfalfa regeneration system was established by studying the influence of explants type, auxins and cytokinins concentration on alfalfa callus induction and differentiation in the experiment. The system was that alfalfa callus were induced on MS ＋ 0. 2mg /L 2,4-D ＋ 0. 01mg /L NAA ＋ 0. 2mg /L KT from leaf; callus was subcultured 2 ～ 3 times and then transferred to MS media for shoot development; and transferred to 1 /2 MS media for roots formation. In the alfalfa culture system,callus induction rate could reach 100% and differentiation rate 90% . The orthogonal experiment of callus induction showed that the major factor on alfalfa callus induction ratio was 2,4-D concentration,the next were explants type,NAA,6-BA and KT concentration. And the factors on alfalfa callus browning rate in the experiment ordinally were explants types,KT,2,4-D,6-BA,NAA. Explants types were significantly effect on callus induction rate and browning rate （P 0. 01）. Auxins （2,4-D and NAA） had greater effect on alfalfa callus induction than cytokinins （6-BA and KT）. The shoot development experiment showed that the hormone component of callus induction media was the determinant factor of alfalfa callus differentiation.
出处
《核农学报》
CAS
CSCD
北大核心
2010年第3期507-512,521,共7页
Journal of Nuclear Agricultural Sciences
基金
中央级公益性科研院所基本科研业务费专项资金资助项目(nyhyzx2007-1-01)
国家科技支撑计划项目(2008BADB3B03)
