摘要
离体培养条件下对紫花苜蓿地方栽培品种———陇东苜蓿、和田苜蓿、准格尔苜蓿以及引进品种Rambler苜蓿植株不同部位的再生进行了研究,结果表明,不同的苜蓿品种、外植体、外源激素种类及其浓度等因素均影响着植株的再生。陇东苜蓿下胚轴愈伤组织出愈率和体细胞胚形成率最高可达91.04%和63.75%;和田苜蓿下胚轴愈伤组织出愈率最高可达92.05%,体细胞胚形成率最高为56.82%。这2个品种在紫花苜蓿的组织培养植株再生中是较理想的试验材料;紫花苜蓿下胚轴是理想的受体材料。苜蓿愈伤组织的诱导、体细胞胚的形成以2.0mg/L2,4 D处理2周为最佳;在2.0mg/L2,4 D+0.5～2.0mg/L6 BA的作用下,外植体产生愈伤组织以及进一步发育的趋势较强。对体细胞胚的发育和幼苗的形成则以2.0mg/L6 BA+0.5mg/LNAA的激素组合进行处理。适宜的生根培养基为1/2MS+1.0mg/LIBA+1%蔗糖+0.7%琼脂。
In vitro plant regeneration in diverse cultivars of Medicago sativa (Longdong, Hetian, Zhungeer and Rambler) and their different donor tissues were studied. The results showed that diverse cultivar,explant and different exogenous hormone and concentration had obvious effect on plant regeneration of Medicago sativa, in which, the cultivar Longdong and Hetian were the optimum genotype for callus induction and somatic embryogenesis. The frequency of callus induction and somatic embryogenesis from hypocotyls of Longdong was (91.04%) and 63.75% respectively, The callus induction rate was as high as 92.05% from hypocotyls of Hetian, it's frequency of somatic embryogenesis was 56.82%. Hypocotyl was the optimum donor tissue for rapid and higher frequency induction of callus and promoted further embryos development in Medicago sativa. All selected cultivars exhibited high frequency in callus induction and somatic embryogenesis on solidified MS medium supplemented with 2.0 mg/L 2,4-D or 2.0 mg/L 2,4-D+(0.5-2.0 mg/L) 6-BA for two weeks, and the later had a better performance. Somatic embryos could develop and plantlets could regenerate on solidified MS medium supplemented with 2.0 mg/L 6-BA or 2.0 mg/L 6-BA+0.5 mg/L NAA. Plants rooted on 1/2 MS medium supplemented with 1.0 mg/L IBA, 1% sucrose and 0.7% agar.
出处
《草业学报》
CSCD
2004年第6期99-105,F003,共8页
Acta Prataculturae Sinica
基金
国家科技部国家转基因植物研究与产业化专项子课题(J00 B 001 12)
国家"十五"重大科技专项(2002BA518A03)资助。
