摘要
以芡欧鼠尾草(Salvia hispanica)成熟种子为外植体,1/2MS为基本培养基,研究不同浓度的外源激素对愈伤组织的诱导、增殖和生根的影响,从而初步建立了芡欧鼠尾草组织培养的快速繁殖体系。结果表明,诱导种子愈伤组织的最适宜培养基为1/2MS+10.0mg·L^(-1) 6-BA+1.0mg·L^(-1) NAA+0.4mg·L^(-1) ZT,诱导率为66.5%;不定芽增殖的最适培养基为1/2MS+3.0mg·L^(-1) 6-BA+0.5mg·L^(-1) NAA,增殖倍数为7.69;生根的最适培养基为1/2MS+0.5mg·L^(-1) NAA,生根率达100%,平均生根数为5.68;移栽后植株生长良好,成活率较高,达97%。
In order to establish the tissue culture and rapid propagation system of Salvia hispanica,the present study explored the impacts of different concentrations of hormone on callus induction,proliferation and root induction with the mature seed of S.hispanica as explant and 1/2MS as the basic medium.The results showed that the optimal medium for callus induction was 1/2MS medium with 10.0mg·L^（-1） 6-BA,1.0mg·L^（-1） NAA and 0.4mg·L^（-1） ZT,which had the highest induction rate of 66.5%.The optimal medium for the proliferation was 1/2MS medium with 3.0mg·L^（-1） 6-BA and 0.5mg·L^（-1） NAA,with the highest multiplication coefficient of 7.69.The optimal medium for the root induction was 1/2MS medium with 0.5mg·L^（-1） NAA,which had the highest root induction rate of 100% and the average rooting coefficient of 5.68.Plants grew very well after transplanting with a survival rate of 97%.
出处
《草业科学》
CAS
CSCD
北大核心
2016年第3期393-399,共7页
Pratacultural Science
基金
湖南省科技计划项目--名贵畅销花卉新品种繁育与产业化开发研究(2014FJ3089)
关键词
芡欧鼠尾草
成熟种子
组织培养
快速繁殖
Salvia hispanica
mature seed
tissue culture
rapid propagation
