摘要
应用妊娠 6～ 8周的人工流产蜕膜组织为材料进行离体人工蜕膜细胞培养 ,观察两种化学结构完全不同的特异性钙调素拮抗剂——三氟拉嗪、蝙蝠葛碱衍生物 ,以及 Ca2 + 螯合剂—— EGTA和 Ca2 +载体—— A2 3187对蜕膜细胞活力的影响。结果表明 :三氟拉嗪、蝙蝠葛碱衍生物与 EGTA对蜕膜细胞活力的抑制作用与药物的浓度及其作用时间密切相关 ,随着浓度加大及作用时间延长而增强。≥ 1 5 μmol/L的三氟拉嗪或≥ 2 5 μmol/L的蝙蝠葛碱衍生物 ,或2 mmol/L的 EGTA均可明显抑制蜕膜细胞活力。三氟拉嗪和蝙蝠葛碱衍生物作用 96小时后分别使细胞活力降至对照组的 8.7%和 1 2 .0 % ,EGTA作用 72小时后降至对照组的 2 8.6%。6μmol/L的 A2 3187可一定程度地刺激细胞活力上升 ,但这种刺激效应随作用时间的延长而逐渐减弱。EGTA可增强三氟拉嗪对细胞活力的抑制作用。由此推测 ,Ca2 + -钙调素系统可能直接参与子宫蜕膜的发育和维持 ,并在其中发挥重要作用。这可能也是钙调素拮抗剂抗早孕的主要机理之一。
The effects of two specific CaM antagonists, trifluoperazine (TFP) and dauricine derivatives (Dd 3), as well as EGTA and A 23187 on the viability of human decidual cells in vitro were studied. The results showed that TFP, Dd 3 and EGTA inhibited the viability of decidual cells in a dose and/or time related manner. The higher concentration and the longer time, more significant the inhibition. TFP (≥15μmol/L), Dd 3 (≥25μmol/L) or EGTA (2mmol/L) obviously inhibited the viability of decidual cells. After 96 hours of culture, TFP and Dd 3 decreased the cell viability to 8 7% and 12 0% of the control, respectively. EGTA decreased the cell viability to 28 6% of the control after 72 hours. A 23187 (6μmol/L) stimulated the cell viability to a certain extent, but the stimulatory effect decreased with time. The presence of EGTA obviously enhanced the inhibition of TFP on the viability of the decidual cells. The results suggest that Ca 2+ CaM system might be directly involved in the development and maintenance of uterine decidua and play an important role in it. This also might be one of the main mechanisms of anti fertility action of CaM antagonists.
出处
《动物学报》
SCIE
CAS
CSCD
1999年第1期80-87,共8页
ACTA ZOOLOGICA SINICA
基金
国家计划生育委员会资助项目
江苏省应用基础资助项目
关键词
钙调素拮抗剂
人体蜕膜
三氟拉嗪
CA^2+
Calmodulin antagonist (CaMA), Human decidual, Trifluoperazine (TFP), Dauricine derivative (Dd 3), Calcium
