摘要
目的 探讨降钙素 (calcitonin ,CT)对人类胚胎着床调节的影响。方法 用不同浓度 (2、5、10nmol/L)的CT ,刺激体外培养的人子宫内膜腺上皮细胞和间质细胞 ,应用粘附细胞仪观察细胞内钙离子 (Ca2 + )浓度变化 ;再用不同浓度 (2、5、10nmol/L)的CT分别作用于人种植前 2细胞期、4细胞期、8细胞期胚胎 ,通过粘附细胞仪检测种植前胚胎荧光相对强度 ,观察细胞内Ca2 + 浓度的动态变化。结果 各种浓度CT对子宫内膜腺上皮细胞内Ca2 + 浓度均无影响 ,而CT可以快速增加间质细胞和种植前胚胎细胞内Ca2 + 浓度 ,并且呈剂量依赖性 ,CT浓度为 10nmol/L时 ,作用 3min ,细胞内Ca2 + 浓度开始增加 ,6min达到高峰 ,4细胞期胚胎开始出现对CT刺激的反应 ,8细胞期胚胎效应最强 ,CT对细胞内Ca2 + 浓度的影响可以持续 2h。结论 种植前胚胎表面和子宫内膜间质细胞上 ,可能存在CT功能性受体 ,CT可能通过加速子宫内膜蜕膜化、促进胚胎生长、分化 。
Objective To observe the role of calcitonin (CT) during the implantation. Methods Human endometrial epithelial cells were cultured. After stimulated with various concentrations of CT, intracellular calcium(Ca 2+)in the epithelial and stroma cells and pre-embryos were measured by the laser scanning confocal microscope. Resutls When stimulated with different concentrations of CT, mean fluorescence levels in the epithelial cells were similar to that of the control. However CT can improve intracellar Ca 2+ of preimplantation embryos and stroma cells in a dose-dependent manner and significantly higher than those of controls. When 10 nmol/L CT was added to the culture medium, the intracellular Ca 2+ concentration in 8-cell embryos rose immediately. Embryo exposure to CT was followed by a series of Ca 2+ bursts that persisted for at least 2 hours. No change in Ca 2+ was observed when culture medium alone was added to the embryos. Pre-loading embryos and stoma cells with the Ca 2+ chelator,prevented the increased fluorescence after CT addition. Conclusions CT play an important role during the procesess of implantation. It maybe improve intracellar of preembryos and accerate the development of preembryos.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2002年第1期16-18,共3页
Chinese Journal of Obstetrics and Gynecology
