摘要
目的研究丹参提取液的体外抑菌作用。方法采用常量肉汤稀释法测定最低抑菌浓度(MIC),探讨其体外抑菌作用。采用杯碟法检测丹参不同提取方式、不同灭菌方式以及改变提取液pH值对金黄色葡萄球菌抑菌活性的影响。结果丹参醇提液对大肠埃希菌、金黄色葡萄球菌、枯草芽胞杆菌、铜绿假单胞杆菌、短小芽胞杆菌均有抑菌作用,其95%醇提液对上述菌的MIC分别为20,20,40,80,80 mg/mL,对白色念珠菌无抑菌作用。丹参醇提液对大肠埃希菌、金黄色葡萄球菌抑菌活性较强;不同灭菌方式及改变提取液pH值对金黄色葡萄球菌抑菌活性无差异。结论丹参提取液具有一定抑菌作用。
Objective To study the antimicrobial activity in vitro of the extract from Salviae miltiorrhizae Ratix et Rhizoma. Methods The minimum inhibitory concentration （MIC） of the extract was determined by the constant broth dilution method, in order to investigate the antimicrobial activity in vitro. The activities of extracts extracted with different extraction methods, different sterilization methods, or at different pH against Staphylococcus aureus were determined by the cylinder-plate method. Results The ethanolic extracts from Salviae miltiorrhizae Radix et Rhizoma had antimicrobial activities against Escherichia coli, S. aureus, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus pumilus, but no inhibitory effect on Blastomyces albicans. The MICs of 95 % ethanolic extract were 20, 20, 40, 80, 80 mg/mL, respectively, which showed that the ethanolic extract had stronger activities against E. coil and S. aureus. There was no difference in antimicrobial activity of ethanolic extracts extracted with different sterilization methods or at different pH against S. aureus. Conclusion The extract from Salviae miltiorrhizae Radix et Rhizoma has antimicrobial activity in vitro.
出处
《食品与药品》
CAS
2010年第5期184-187,共4页
Food and Drug
关键词
丹参
最低抑菌浓度
抑菌作用
Salviae miltiorrhizae Radix et Rhizoma
minimum inhibitory concentration
antimicrobial activity
