摘要
研究胆囊结石病人Apo B基因中Xba I位点的多态性及其与血脂的关系。方法:对190例胆囊结石病人组和442例正常人对照组,采用PCR-RFLP法分析Xba I多态性的基因型,测定血脂。结果:胆石组的X^(+/-)基因型频率显著高于对照组(男性:16.7％比8.0％,P=0.01;女性:26.7％比7.7％,P<0.001;总体:20.6％比7.9％,P<0.001)。胆石组的X^+等位基因频率显著高于对照组(男性:8.33％比4.01％,P<0.05;女性:14.47％比3.85％,P<0.001;总体:10.79％比3.96％,P<0.001)。胆石组携带X^+等位基因者的血清总胆固醇(4.85±0.75mmol/L比4.54±0.89mmol/L,P<0.05)和低密度脂蛋白胆固醇(2.74±0.58mmol/L比2.49±0.69mmol/L,P<0.05)均高于不携带X^+等位基因者,对照组携带X^+等位基因者的血脂也有类似的增高趋势。结论:Apo B的X^+等位基因有增高血清胆固醇和低密度脂蛋白胆固醇的特点,可能是胆固醇结石形成的易感基因。
To study the distribution of Apo B gene Xba I polymorphism in patients with gallbla-dder stones and the changes in their serum lipids. Methods: Xba I genotypes were measured by PCR-RPLP, and the serum lipids assayed in 190 patients with gallbladder stones and in 442 normal patients as controls. Results: The frequency of X+/- genotype was significantly higher in patients with gallbla-dder stone(s) than that in controls(male:16.7% vs. 8.0%, P=0.01; female:26.7% vs. 7.7%, P<0.001; total: 20.6% vs. 7.9%, P<0.001). There was also a higher frequency of X+ allele in the group of patients with gallbladder stone(s) as compared with the control group (male:8.33% vs. 4.01%, P<0.05; female:14.47% vs. 3.85%, P<0.001; total:10.79% vs. 3.96%, P<0.001). The patients with X+ allele had a significantly higher total serum cholesterol (4.85±0.75 mmol/L vs. 4.54±0.89 mmol/L, P<0.05) and higher low de-nsity lipoprotein cholesterol(2.74±0.58 mmol/L vs. 2.49±0.69 mmol/L, P<0.05) than those in patients without X + allele. There was a similar tendency of increase in serum cholesterol and low density lipo-protein cholesterol in carriers of X+ allele of the control group. Conclusions: The X+ allele of Apo B gene is associated with a higher serum cholesterol and higher low density lipoprotein level and it may be regarded as a high-risk gene of cholesterol gallstone disease.
出处
《外科理论与实践》
1999年第1期18-21,共4页
Journal of Surgery Concepts & Practice
基金
上海市科技发展基金(954119027)资助
关键词
胆结石
载脂蛋白B
基因多态性
血脂
Gallbladder stone Apolipoprotein B Gene polymorphism
