摘要
目的:研究肝脏肝X受体α(liverXreceptorα,LXRα)和雌激素受体α(estrogenreceptorα,ERα)表达与女性胆石病发生的关系。方法:采用定量PCR方法测定11例女性胆囊胆固醇结石患者和10例胆囊息肉患者肝脏LXRα、ERα以及胆小管侧膜ABC基因(ABCG5,ABCG8,ABCB11,ABCB4)的表达差异。结果:胆石病组患者LXRα基因表达较对照组增加31%,差异显著(P<0.05);ERα表达增加35%,与对照组比较差异无显著性(P>0.05)。ABCG5和ABCG8分别增加53%和64%,差异显著(P<0.05)。ABCB11和ABCB4表达无差异。结论:肝脏LXRα表达增加刺激ABCG5和ABCG8表达增加,从而促进胆汁胆固醇分泌是女性胆石病发生因素之一。ERα增加也可能参与了女性胆石病的发生,有待进一步研究。
Objective To compare the difference of hepatic liver X receptor α （LXRα） and estrogen receptor α （ERα） expression between female cholesterol gallstone patients and gallstone-free controls. Methods Gene expression of LXRα, ERα and canalicular membrane transporters （ABCG5, ABCG8, ABCB11 and ABCB4） were assayed by real-time PCR between 11 female cholesterol gallstone patients and 10 female gallstone-free controls. Results LXRα increased 31% in the gallstone patients in comparison with gallstone-free controls （P〈0.05）. ERα increased 35% in the gallstone patients without significance in comparison with controls （P〉0.05）. Both ABCG5 and ABCG8 increased significantly in the gallstone patients. Neither of ABCBI 1 and ABCB4 differed between the two groups. Conclusions From this study exclusively in the females, it shows that increased LXRα up-regulating ABCG5 and ABCG8 is one defect that leads to hypersecretion of biliary cholesterol and formation of gallstone. ERα may play a role in the formation of gallstone disease in the females, but it requires further study.
出处
《诊断学理论与实践》
2006年第4期308-311,共4页
Journal of Diagnostics Concepts & Practice
基金
国家自然科学基金(No.30271272)
