摘要
从广东发病鸽群中采集的拭子样本中分离到1株新城疫病毒,用聚合酶链反应(PCR)、血凝抑制试验(HI)及基因测序等对其进行鉴定。结果表明,此分离株具有血凝活性,且可与新城疫病毒标准阳性血清发生特异性抑制反应;用针对新城疫病毒的F基因及P基因特异性鉴定引物对该分离株进行PCR扩增,可扩增出特异性目的片段;测序及BLAST分析表明,其与美国新城疫病毒分离株Pigeon/US(TX)/98的F基因核苷酸序列相似性高达97%以上。毒株被鉴定为鸽源新城疫病毒,并命名为Pigeon/Guangdong/ZQ-17。
A Newcastle disease virus（NDV） virus was isolated from pigeon in Guangdong.It was identified with PCR,hemagglutination inhibition and sequence analysis.The results showed the isolate had hemagglutination and was inhabited by the standard antibody of NDV;it was amplified by RT-PCR,which was used by the specific identification primers of F,P of NDV,and gained the specific segments.Blasted and compared with NDV of GenBank in NCBI,the F gene of the isolate shared the closed similarity with the NDV from USA,and the similarity was over 97%.Thus,the isolate was identified as a pigeon origin NDV,named Pigeon/Guangdong/ZQ-17.
出处
《动物医学进展》
CSCD
北大核心
2010年第5期23-25,共3页
Progress In Veterinary Medicine
基金
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723)
公益性行业(农业)科研专项经费项目(200803020-08)
广东省科技计划项目(2004B20201030
5500E08020
2007A020100006)
关键词
新城疫病毒
鸽
分离鉴定
NDV
pigeon
isolation identification
