摘要
cDNA末端快速扩增—RACE(rapid amplification of cDNA ends),是近年发展起来的快速有效的获得全长cDNA的途径之一。本文阐述了RACE的历史、技术原理、局限性以及新型的RACE技术,并且讨论了RACE技术在植物基因研究中的技术优势、应用现状和发展前景。
RACE(rapid amplification of cDNA ends),is one of the rapid and efficient way to get the full-length cDNA,which has developed in recent years.The article has been summed up the technical principle,limitation,method improvement of RACE and new style of RACE technique clearly.And the technical advantatg,application status and development prospect of RACE technique in the research on plant gene were discussed.
出处
《齐齐哈尔大学学报(自然科学版)》
2010年第3期65-68,共4页
Journal of Qiqihar University(Natural Science Edition)
基金
黑龙江科技厅农业攻关项目
黑龙江省教育厅海外学人合作项目(1151hz022)