摘要
根据已知的几种十字花科植物几丁质酶基因保守序列设计特异性引物,以大白菜叶片基因组DNA为模板,通过PCR反应扩增出约1kb的DNA片段和几丁质酶基因5′端序列片段(150bp)。利用不同浓度的水杨酸处理苗龄7d的大白菜,选择酶活力较高的处理植株叶片提取RNA,采用RACE技术扩增。研究结果表明,CHB4基因的DNA序列长度为1517bp(DDBJ登录号:AB257452),包含有1个长度为508bp内含子。该基因编码的产物是由268个氨基酸残基组成的蛋白质,氨基酸序列与油菜ClassⅣ类几丁质酶的同源性达99%,与其它几种高等植物的同源性达50%以上。
Based on the conserved sequence of the ehitinase of erueifers, about 1 kb DNA fragment and 5＇-end fragment （150 bp） were amplified by PCR from genomic DNA of Chinese cabbage. Experiments were performed on 7-day-old Chinese cabbage seedlings after germination with salicylic acid of different concentrations. Total RNA was prepared from the plant with higher chitinase activity, and 3＇- end fragment was amplified by 3＇ RACE. Comparing Chinese cabbage chitinase sequence cloned in this experiment with that reported in GenBank composed of 1 094 bp nucleotide of Brassica napus, the experiment showed that the chitinase sequence of Chinese cabbage is 1 517 bp in size with one intron of 508 bp （DDBJ accession number： AB257452）, and shares as high as 99% identity with B. napus and over 50% with other high plants in cod- ing region.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第1期105-110,共6页
Acta Horticulturae Sinica
基金
安徽省教育厅自然科学研究项目(2006KJ211B)
