摘要
目的探讨合肥地区女性子宫颈感染人乳头瘤病毒(HPV)的基因型分布和感染率情况。方法采用核酸分子快速导流杂交基因芯片技术(HybriMax)对妇科阴道镜门诊506例患者进行HPV21分型检测,阴道镜下宫颈多点活检并经病理证实病变程度。结果①HPV总的阳性率为46.6%(236/506),宫颈病变程度加重,HPV阳性率逐渐升高(25.0%～100.0%)。最常见的高危感染亚型为HPV16(50.0%)和52(26.7%),其次是33(11.4%),58(8.5%),18(6.4%);②HPV阳性患者单一感染和多重感染率分别为62.7%(148/236)和37.3%(88/236),多重感染中主要以二重感染为主86.4%(76/88),尤其以16和52型为主。CIN2-3组多重感染比例较高,与正常和炎症组、CIN1组和宫颈癌组比较有统计学意义(P<0.05),宫颈癌患者则以单一感染多见。结论合肥地区女性子宫颈感染HPV最常见的亚型为16、52、33、58和18。二重感染尤其是HPV16/52联合其他亚型与宫颈CIN2-3密切相关,而与宫颈癌关系不大。
Objective To determine the infection rate of HPV and genotypes distribution in Chinese women of Hefei.Methods A total of 506 colposcopy outpatient women were checked for 21 HPV DNA types by DNA Flow-through Hybridization and Gene Chip,and cervical biopsies were used for pathological diagnosis.Results ① The overall HPV prevalence was 46.6%（236/506）,with cervical lesion severity,HPV positive rate were increased （25.0% ～100.0%）.The most common high risk types were HPV16（50.0%）and 52（26.7%）,followed by 33 （11.4%）,58（8.5%）and 18（6.4%）;② Single and multiple infection rate were 62.7%（148/236）and 37.3%（88/236）,respectively.Dual infection was main type of multiple infection,the most common models was HPV16/52 combined with other types.CIN2-3 multiple infection rate was higher compared to negative or cervix,CIN1 and cervical cancer（P0.05）.Single infection was common in cervical cancer.Conclusion HPV16,52,18,33 and 58 are the most prevalence high-risk subtypes in Hefei.Double infection especially HPV16/52 in conjunction with other subtypes is associated with CIN2-3,but not cervical cancer.
出处
《中国临床保健杂志》
CAS
2010年第2期141-144,共4页
Chinese Journal of Clinical Healthcare
基金
安徽省自然科学基金项目(090413117)
关键词
宫颈疾病
乳头瘤病毒感染
宫颈上皮内瘤样病变
横断面研究
Uterine cervical diseases
Papillomavirus infections
Cervical intraepithelial neoplasia
Cross-sectional studies
