摘要
以大豆内源基因(Lectin)、筛选基因35S启动子(Cauliflower mosaic virus 5S,CaMV35S)、NOS终止子(Nopaline synthase,Nos)和外源基因(CP4 EPSPS)为检测对象,对大豆深加工产品的DNA提取方法、PCR扩增条件的退火温度、引物浓度进行探讨,得出不同DNA提取方法、退火温度、引物浓度对大豆加工产品PCR检测的影响,建立了大豆加工食品中转基因成分定性检测体系。检测结果表明,退火温度60℃、引物浓度0.4μM时所建立的定性PCR检测方法能有效检测出大豆加工产品中的转基因成分,而且方法简单、快速有效。
The purpose of this study is to evaluate the impact of factors on detesting genetically modified components in intensive processing soybean products. The products were investigated by PCR detection of lection,CaMV35S promoter, NOS terminators and CP4 EPSPS. In this research, an easy, fast and effective method was established by analyzing the various factor. The detection results show that the most optimal annealing temperature and primer concentration was 60℃ and 0.4 μM.
出处
《农业科技与装备》
2010年第3期31-33,共3页
Agricultural Science & Technology and Equipment
关键词
转基因
大豆加工产品
定性检测
PCR
genetically modified organisms, intensive processing soybean product, qualitative detection PCR
