摘要
近年来转基因食品定性检测方法发展迅速,然而目前转基因成分GMO的准确定量检测在国际贸易中日趋重要。我们这里介绍三种定量检测方法:半定量PCR法,定量竞争PCRQC-PCR法和实时定量PCR法。半定量PCR法比较简单,但结果不是很精确。定量竞争PCR的特点是含有内部标准子。近来开展的实验室合作研究表明,与定性PCR法相比,定量竞争PCR降低了实验室之间的误差。而实时定量PCR法可在提取DNA后3h内,测出每克起始样品的总DNA量及2pg转基因成分的量,但这套PCR系统目前价格昂贵。
Genetically modified food qualitative detection methods have evolved f ast during the past years.However,t he exact quantification detection of t he presence of genetically modified organisms (GMO)has become very important in in-ternational commercial transactio ns.We introduce three kind of quantitative detection methods:semi -quantitative,quantitative competitive PRC(QC -PCR)and real -time quantitative PCR.Sem i -quantitative method is comparative convenient method,but its result is nt accurate.Quantitative competi tive PCR(QC -PCR)is characterized by internal standards.A recently performed collaborative study demonstrated that QC -PCR led to comparable determinations of the GMOcontent of food samples reducing existing inter -laboratory differe nces(comparing with qualitative PCRmeth od).Using real -time quantitative PCR,2pg of t ransgenic or total DNA per gram of sta rting sample was detected in 3hours a fter DNA extraction.But this kind of PCR s ystem is expensive at present.
出处
《食品科技》
CAS
北大核心
2001年第5期63-64,62,共3页
Food Science and Technology
