摘要
目的研究角膜移植免疫排斥反应的免疫病理学变化及免疫细胞和某些相关免疫分子在角膜移植免疫排斥反应中的作用，阐明ＦＫ５０６的免疫抑制机理。方法应用免疫组织化学染色方法检测ＦＫ５０６、环胞霉素Ａ及对照组角膜植片中ＣＤ＋４、ＣＤ＋８、巨噬细胞、白细胞介素－２受体、Ⅱ类主要组织相容性抗原、细胞间粘附分子－１及淋巴细胞功能相关抗原－１的表达。结果排斥的角膜组织大量表达上述免疫细胞和分子，ＦＫ５０６可对此产生明显的抑制作用。结论迟发型超敏反应和细胞毒性Ｔ细胞作用与植片的免疫性损伤密切相关，免疫粘附分子，Ⅱ类主要组织相溶性抗原的异常表达在排斥反应中具有重要作用。
Objectives To study the immunopathologic process of rejection, the effect of certain immune cells and molecules on rejection and elucidate the immunosuppressive mechanism of FK 506. Methods By using immunohistochemical techniques, the expression of CD + 4 cells, CD + 8 cells, macrophages, IL 2 receptor, MHC Ⅱ antigen, intercellular adhesion molecule 1 (ICAM 1) and lymphocyte function associated antigen 1 (LFA 1) in rejected corneal grafts were studied. Results Immunohistochemical studies showed diffuse infiltration of CD + 4 cells, CD + 8 cells and macrophages, and strong expression of IL 2 receptor, MHC Ⅱ antigen, ICAM 1 and LFA 1 in rejected corneal grafts, especially in the acute rejection stage. FK 506 can inhibit the expression of these cells and molecules in the grafts. Conclusion Keratoplasty rejection is significantly related to delayed type of hypersensitivity and cytotoxic T cells, and immune adhesion molecules and MHC Ⅱ antigen also affect the rejection process.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1998年第1期21-24,I001,共5页
Chinese Journal of Ophthalmology
