摘要
目的：研究角膜移植排斥反应的免疫病理学变化，阐明Ｌｅｆｌｕｎｏｍｉｄｅ的免疫抑制机理。方法：应用免疫组织化学染色方法检测环孢霉素Ａ、Ｌｅｆｌｕｎｏｍｉｄｅ及对照组角膜移植片中ＣＤ４＋细胞、ＣＤ８＋细胞、白细胞介素－２受体及主要组织相容性Ⅱ类抗原的表达。结果：排斥的角膜组织大量表达上述免疫细胞和分子，Ｌｅｆｌｕｎｏｍｉｄｅ可以对这些细胞和分子产生明显的抑制作用。结论：迟发型超敏反应和细胞毒性Ｔ细胞的作用与植片的免疫性损伤密切相关，白细胞介素－２受体和主要组织相容性Ⅱ类抗原的异常表达在排斥反应中具有重要的作用，Ｌｅｆｌｕｎｏｍｉｄｅ能抑制淋巴细胞的活化、增殖，延长角膜移植物的存活时间。
To study the immunopathologic process of rejection and elucidate the
immunosuppressive mechanism of leflunomide By using immunohistochemical
techniques,the expression of CD4 + cells,CD8 + cells,IL-2 receptor,MHC-Ⅱ antigen in
rejected corneal grafts were studied Immunohistochemical studies showed diffuse
infiltration of CD4 + cells,CD8 + cells and strong expression of IL-2 receptor and MHC-
Ⅱ antigen in rejected corneal grafts,especially in the acute rejection stage Leflunomide
can inhibit the expression of the cells and molecules in the grafts Keratoplasty rejection
is significantly related to delayed type of hypersensitivity and cytotoxic T cells,and IL-2
receptor and MHC-Ⅱ antigen also affect the rejection process;Leflunomide prolongs the
survival time of corneal allografts by inhibiting T cell activation and proliferation
出处
《眼科》
CAS
1999年第3期168-171,共4页
Ophthalmology in China
关键词
角膜移植
穿透性
移植物排斥
免疫组化
Keratoplasty,penetrating
Graft rejection
Immunohistochemistry
Isoxazoles/analogs
