摘要
以广东德庆何首乌为试材,研究叶片的愈伤组织诱导分化及茎尖的增殖快繁技术。结果表明:以MS为基本培养基,2,4-D是诱导愈伤组织的必需激素,1～2 mg/L有利于叶片愈伤组织诱导;分化培养基BA1～2 mg/L+NAA0.5～1 mg/L浓度组合分化能力低,未分化成芽;培养基BA2 mg/L+NAA0.5 mg/L对茎尖不定芽的诱导效果较好;生根培养基1/2 MS,生根率100%。
This study was to set up the methods for tissue culture and stem rapid propagation in vitro of Polygonum mul- tiflorum Thunb. The results showed that 2,4-D was the necessary hormone in MS medium in order to induce callus from leaf, and the suitable concentration of 2, 4-D was 1.0~2.0 mg/L. The MS medium containing 1~2 mg/L BA and 0. 541 mg/L NAA can not effectively give rise to callus from leaf, but the MS medium containing 2 mg/L BA and 0. 5 mg/L NAA can significantly promote the stem rapid propagation in vitro. The root inducing medium was 1/2 MS, and the rooting rate was 100%.
出处
《北方园艺》
CAS
北大核心
2010年第7期175-177,共3页
Northern Horticulture
基金
广州大学科研创新团队资助项目
关键词
何首乌
道地药材
组织培养
快繁
Polygonum multiflorum Thunb.
genuine medicine material
tissue culture
rapid propagation
