摘要
采用单因子、正交设计法考察了基本培养基、激素以及光暗条件对何首乌幼嫩茎、叶诱导愈伤组织的影响,以及培养时间与愈伤组织生长量间的关系。实验研究表明:MS+2,4D1mg/L+6-BA1mg/L+IBA0.5mg/L和暗培养对诱导何首乌愈伤组织产生效果较好;接种12天时愈伤组织生长量达到最大;愈伤组织经诱导能够产生完整植株,成苗率为95％。
In the present thesis, suitable medium for callus inducement and relationship between culture time and callus growth of Poligonum multiflorum were studied by mono-factorial and orthogonal design test.The experiments showed that the best medium for callus inducement was MS + 2,4 -D lmg/L + 6-BA lmg/L + IBA 0.5 mg/L, dark culture was better than light culture, callus grew fast when it was cultured 12 days, whole plant could be induced by callus, the induce rate was 95%.
出处
《中药材》
CAS
CSCD
1998年第3期109-110,共2页
Journal of Chinese Medicinal Materials
关键词
何首乌
组织培养
愈伤组织
诱导
植株
Poligonum multiflorum
Tissue culture
Callus
Inducement
Plant
