摘要
研究Picloram、ABA和TDZ对贡蕉未成熟花序来源的胚性细胞悬浮系体细胞胚胎发生的影响。结果表明,8.28μmol/L Picloram替代2,4-D可获得15.56%的胚性愈伤组织诱导率;在体细胞胚诱导初期加入ABA则抑制体细胞胚的发生,并造成其萌发时严重愈伤化;TDZ可有效地改善体胚萌发率和植株转换率,其中0.2μmol/L TDZ处理的体胚萌发率和植株转换率分别从对照的17.28%、16.49%提高到72.77%和67.05%。
Establishment of a stable embryogenic cell suspension （ECS） is a prerequisite for biotechnological breeding of banana. Although many important progress has been made, but application of embryogenic cell suspension for genetic improvement of Musa is limited because of low induction percentage of embryogenoic callus, low conversion frequence of plant regeneration from the somatic embryos. Therefore, an optimal protocol for embryogenic cell suspensions is still required to establish. In this study the effects of Picloram, ABA and TDZ on somatic embryogenesis of banana from young male flower of Musa acuminata cv. Mas （AA） were investigated. Results showed that the induction percentage of embryogenic calli reached 15.6% when 2,4-D in the callus induction medium was substituted by 8. 28 μmol/L picloram. However, the addition of ABA into somatic embryo development medium inhibited somatic embryogenesis from embryogenic cell suspension and resulted in callus formation from somatic embryos. Treatment with TDZ at concentration of 0. 2 μmol/L resulted in increase of the percentage of germination of somatic embryos from 17.28% to 72. 77% and the plant conversion ratio from 16. 49% to 67.05%, respectively.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第1期81-86,共6页
Acta Horticulturae Sinica
基金
广东省自然科学基金资助项目(031995
5300966)
广东省科技计划项目(2005B20901017)
广东省农科院博士启动项目(2005008)
