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基于SRAP分子标记的海南沼虾种群遗传多样性 被引量:14

Genetic diversity in five Macrobrachium hainanense populations using SRAP markers
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摘要 为了调查我国海南沼虾(Macrobrachium hainanense)不同地理种群遗传多样性及遗传分化状况,本文采用序列相关扩增多态性(sequence-related amplified polymorphism,SRAP)标记对我国南方瓯江(OJ)、闽江(MJ)、珠江(PR)、万泉河(WQ)、昌化江(CH)等5个海南沼虾种群的遗传多样性进行了研究。共得到255个清晰、稳定的位点,平均多态位点比例为47.05%,由小到大依次为:PR(43.92%)=WQ(43.92%)<MJ(46.67%)<CH(47.45%)<OJ(53.33%)。遗传杂合度由小到大依次为:PR(0.1657)<WQ(0.1763)<CH(0.1799)<OJ(0.1839)<MJ(0.1892),平均为0.1790。Shannon信息指数由小到大依次为:PR(0.2543)<WQ(0.2658)<CH(0.2746)<MJ(0.2846)<OJ(0.2876),平均为0.2734。AMOVA分析结果显示,17.64%的遗传变异来自种群间,对总遗传变异影响显著(P<0.001)。遗传分化指数(Gst)、基因流(Nm)、遗传距离和聚类分析结果均显示5个海南沼虾种群间已经出现较大的遗传分化。 We studied the genetic variation and differentiation of five wild populations of Macrobrachium hainanense using SRAP markers.Samples were collected from Oujiang River(OJ), Minjiang River(MJ), Pearl River(PR), Wanquan River(WQ) and Changhuajiang River(CH).A total of 255 loci were detected, of which 47.05% were polymorphic.Proportion of polymorphic loci of the five populations ranked in the order:PR(43.92%) = WQ(43.92%) MJ(46.67%) CH(47.45%) OJ(53.33%), while heterozygosity ranked as PR(0.1657) WQ(0.1763) CH(0.1799) OJ(0.1839) MJ(0.1892), with an average of 0.1790.Shannon index ranked as PR(0.2543) WQ(0.2658) CH(0.2746) MJ(0.2846) OJ(0.2876), with an average of 0.2734.Analysis of molecular variation(AMOVA) revealed that 17.64% of the variance was among populations, which significantly affected the total variance(P 0.001).Values of Gst, Nm and genetic distance suggested the genetic divergence among populations was relatively high(P 0.001).
出处 《生物多样性》 CAS CSCD 北大核心 2010年第2期145-149,共5页 Biodiversity Science
基金 国家"十一五"科技支撑计划(2006BAD01A13) 农业部农业科技跨越计划(2007年度) 江苏省高技术研究项目(BG2007328)
关键词 MACROBRACHIUM hainanense SRAP 遗传多样性 遗传分化 种群遗传结构 Macrobrachium hainanense SRAP genetic diversity genetic differentiation population structure
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