摘要
利用SRAP分子标记技术对陕西省的144株油菜菌核病菌(Sclerotinia sclerotiorum(Lib.)de Bary)和18株不同寄主来源的菌核病菌进行遗传多样性分析。从150对SRAP引物中筛选得到9对多态性高、稳定性好的引物组合,对不同地区的144株菌株进行PCR扩增共得到76条多态性条带,多态性比例为88.4%,相似系数为0.346～0.936。对不同寄主的18株菌株扩增后得到多态性条带62条,占总数的84.9%;相似系数为0.358～0.976。UPGMA聚类分析显示,不同地区的144株菌在相似系数0.658处被划分为4个类群,第Ⅰ类为汉中类群,主要菌株来源为陕南汉中各县,第Ⅳ类为安康类群,主要菌株来源为陕南安康2县,第Ⅱ与Ⅲ类为混合类群;不同寄主的18株菌在相似系数0.788时被聚为3个类群,其中83.3%的菌株聚在同一类群中但来自不同供试寄主;已知致病力的38株菌在相似系数0.730处被分为3个类群,A类群主要由相对较弱的致病力菌株组成,而B与C类群为致病力混合类群。以上研究结果表明,陕西省油菜菌核病菌存在丰富的SRAP多态性,其遗传多样性与地理来源为总体相关性不明显,与寄主种类及致病力差异也无显著相关性。
We analyzed genetic diversity of 144 Sclerotinia sclerotiorum isolates from different areas and 18 isolates from different hosts in Shaanxi province using sequence-related amplified polymorphism（SRAP）markers.Nine primer pairs were screened out from 150 SRAP primer combinations based on their polymorphism and stable amplification.In this study,76 polymorphic bands were produced by the amplification of 144 isolates,which was 88.4% of the total bands,and the similarity coefficient was from 0.346 to 0.936.Meanwhile,with the amplification of 18 strains from different hosts,we got 62 polymorphic bands which made up 84.9% of the total bands,and the similarity coefficient was from 0.358 to 0.976.The UPGMA（Unweighted Pair Group Method With Arithmetic Mean）cluster analysis showed that the dendrogram consisted of four groups when the genetic similarity coefficient of 144 strains in different regions was 0.658.Group Ⅰ consisted of counties mainly in Hanzhong,while Group Ⅳ consisted of 2 counties in Ankang.Group Ⅱ and Ⅲ are groups from mixed regions.Meanwhile,the dendrogram consisted of three groups when the genetic similarity coefficient of 18 strains in different hosts was 0.788,and 83.3% of them were in groupⅠ consisting of all host types.The dendrogram consisted of three groups when the genetic similarity coefficient of 38 strains of different pathogenicity was 0.730.They are relatively weak pathogenicity isolates in group A,combined pathogenicity in B and C.Results showed a rich SRAP polymorphism among the population of S.sclerotiorum in Shaanxi province but had no significant correlation with genetic diversity and geographical location,nor did the isolates in different host origin and pathogenicity.
出处
《西北农业学报》
CAS
CSCD
北大核心
2012年第10期149-155,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
公益性行业(农业)科研专项(201103016)
高等学校学科创新引智计划(B07049)
关键词
油菜菌核病
核盘菌
遗传分化
聚类分析
致病力
Rape Sclerotinia rot
Sclerotinia sclerotiorum
Genetic differentiation
Cluster analysis
Pathogenicity
