摘要
为了优化怀地黄多糖的微波提取工艺,采用苯酚—硫酸比色法对怀地黄多糖含量进行测定,通过设定不同单因素研究了微波处理时间、微波功率和液料比对怀地黄多糖提取率的影响,以响应面分析法确定了微波提取怀地黄多糖的最佳工艺。结果显示:微波处理时间、微波功率和液料比对地黄多糖得率具有显著影响,其中在微波处理功率为720W,微波处理时间为210s,料水比为1:6条件下,地黄多糖提取率可达到61.9%,并且该工艺有很好的重现性。
In order to obtain the optimum condition of the Rehmannia glutinosa Libosch polysacchafide microwave-assisted extraction techniques, the content of polysaccharide in Rehmannia glutinosa Libosch was measured by phenol-H2SO4 eolorimetric method. This paper studied the effect of treatment time of microwave, power of microwave and rate of water to material through devised different factors. The optimum microwave extraction condition was determined by response surface analysis. The results showed the treatment time of microwave, power of microwave and the rate of material to material had a remarkable effect on obtain of polysaccharide in Rehmannia glutinosa Libosch. When the power of microwave was 720W, the treatment time of microwave was 210s, the rate of material to water was 6：1, the extraction rate of polysaccharide in Rehmannia glutinosa Libosch was 61.9%. This result also had a good reproducibility.
出处
《中国农学通报》
CSCD
北大核心
2010年第9期60-64,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"阿魏酸影响地黄块根膨大机理研究"(30671201)
河南农业大学创新基金"地黄对芝麻的化感作用研究"(2009-CX-017)
关键词
怀地黄
多糖
微波提取
响应面
Rehmannia glutinosa Libosch
polysaccharide
microwave extraction
the response surface
