摘要
目的:探讨胰岛素样生长因子-I受体(IGF-IR)和蛋白激酶C(PKC)在喉鳞状细胞癌中的表达情况及临床意义。方法:采用免疫组织化学SP法及Westernblot技术检测60例喉鳞状细胞癌组织、25例癌旁组织及10例正常喉部组织中IGF-IR及PKC的表达情况。结果:IGF-IR及PKC在喉鳞状细胞癌中的表达阳性率较正常喉部组织及癌旁组织明显增高(P<0.05)。IGF-IR在TNM分期Ⅲ～Ⅳ期、颈淋巴结转移及高分化癌患者中的表达水平分别较Ⅰ～Ⅱ期、无淋巴结转移及中低分化癌患者表达增强(P<0.05)。PKC的阳性表达与喉癌临床分期及淋巴结转移相关(P<0.05)。喉鳞状细胞癌组织中IGF-IR与PKC蛋白的表达呈正相关(rs=0.338,P<0.05)。结论:IGF-IR及PKC在喉鳞状细胞癌中表达增高且两者密切相关,可能参与了喉癌的发生、发展过程,检测IGF-IR和PKC的表达对喉癌的早期诊断及治疗具有重要指导意义。
Objective：To investigate the expression of IGF-IR and PKC and their clinical significance in laryngeal squamous cell carcinoma（LSCC）.Method：The expression of IGF-IR and PKC protein were detected by immunohistochemistry and Western blot in 60 cases of LSCC,25 cases of adjacent non-tumorous laryngeal epithelium and 10 cases of normal laryngeal epithelium.Result：The positive rate of IGF-IR and PKC expression in LSCC were significantly higher than that in normal laryngeal epithelium and adjacent non-tumorous laryngeal epithelium（P〈0.05）. IGF-IR positive expression were increased more in cases with stage Ⅲ-Ⅳ,cervical lymph node metastasis and well differentiation than that in cases with stageⅠ-Ⅱ,non-lymph node metastasis and poor/mediate differentiation,respectively（P〈0.05）.The positive rate of PKC in LSCC were correlated with clinical stage and cervical lymph node metastasis.There was a correlation between the expression of IGF-IR and PKC in LSCC.Conclusion：The overexpression of IGF-IR and PKC in the laryngeal carcinoma may play an important role in the carcinogenesis and development of LSCC. It is suggested that detecting the expression of IGF-IR and PKC can be used for early diagnosis and treatment of LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2010年第4期152-155,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
胰岛素样生长因子-Ⅰ受体
蛋白激酶C
喉肿瘤
癌
鳞状细胞
insulin-like growth factor-I receptor
protein kinase C
laryngeal neoplasms
carcinoma
squamous cell
