摘要
目的建立四味姜黄胶囊中姜黄素含量的RP-HPLC测定方法。方法采用Shim-pack C18柱,以乙腈-0.5%冰醋酸溶液（45∶55）为流动相,流速为1.0mL.min-1,检测波长为427nm。结果姜黄素浓度在1.9698.0μg.mL-1范围内与其峰面积呈良好的线形关系（r=0.9999）。平均回收率为98.5%（n=9）,RSD为1.6%。结论本方法操作简便、快速、准确,可用于四味姜黄胶囊中姜黄素的含量测定。
Objective To establish an RP-HPLC method for the determination of curcumin in Siwei Jianghuang Capsules.Methods The separation was performed on Shim-pack C18 column using acetonitrile-0.5% glacial acetic acid（45∶55）as mobile phase.The flow rate was 1.0mL·min-1.The detection wavelength was 427nm.Results The linear range of curcumin was within 1.9698.0μg · mL-1（r=0.9999）.The average recovery was 98.5%（n=9）and RSD was 1.6%.Conclusion The method is simple,fast and accurate for analysis of curcumin in Siwei Jianghuang Capsules.
出处
《中国药事》
CAS
2010年第2期170-171,181,共3页
Chinese Pharmaceutical Affairs
