摘要
目的观察3种不同保护剂对液氮冻存同种带瓣大动脉组织结构影响,寻求最佳冷冻保护剂。方法单独使用0.1mol/L二甲亚砜(对照组)及单独使用0.1mol/L海藻糖(实验组1)和0.1mol/L二甲亚砜+0.1mol/L海藻糖(实验组2)作为冷冻保护剂,用光镜及电镜对新鲜组织及液氮保存6、9、12个月的带瓣大动脉进行形态学观察。结果随着保存时间的延长,3组结构破坏均逐渐加重。光镜下观察,保存超过6个月后,3组形态学与新鲜组织比较均有改变(d=17～30,P<0.01),3组之间比较差异有显著意义(d=6～13,P<0.01、0.05)。电镜下观察,保存6、9、12个月时,对照组的同种带瓣大动脉组织的超微结构改变十分明显,实验组1改变较对照组轻,实验组2的结构改变最轻。结论海藻糖对液氮保存的带瓣大动脉有较好的保护作用;单独使用0.1mol/L海藻糖保护效果优于单独使用0.1mol/L二甲亚砜,二者联合应用保护效果明显好于单独使用。
Objective To compare the effects of three cryoprotectants on the structure of allogenetic aortic valves preserved in liquid nitrogen （LN） and search for the optimal one. Methods The allograft preserved in 0. 1 mol/L DMSO served as control group; in 0. 1 mol/L trehalose as experiment group 1, and in 0.1 mol/L DMSO plus 0.1 tool /L trehalose as experiment group 2. The morphology of the fresh tissue and that had been preserved by LN 6, 9 and 12 months was observed light-microscopically and electron-microscopically. Results The structures of the tissues in the three groups were increasingly destroyed with prolongation of preservation duration. Light-microscopically, the morphology of the tissues in the three groups after six months of preservation was changed as compared with that of fresh tissues （d= 17--30, P〈0. 01）. The differences among the three groups were significant （d= 6-13;P〈0.01,0. 05）. Electron-microscopically, the changes of the ultramicrostructure of the aortic tissue in the control group were dramatically after 6, 9 and 12 months of preservation, the changes in group 1 was milder, and that in group 2, the mildest. Conclusion Trehalose is a good cryoprotectant for the allogenetic aortic valve preserved in the LN. The cryoprotective effect of trehalose combined with DMSO is better than that of trehalose and DMSO alone.
出处
《青岛大学医学院学报》
CAS
2010年第2期109-112,共4页
Acta Academiae Medicinae Qingdao Universitatis
基金
青岛市科技发展计划项目(06-2-2-6-nsh-1)
关键词
同种瓣
低温保存
海藻糖
allogenetic valve
cryopreservation
trehalose
