摘要
本实验以绿茶为原料对两种茶类胡萝卜素的高效液相色谱样品制备方法进行了比较研究。结果表明：采用已烷：丙酮：乙醇：甲苯（10：7：6：7）与40％甲醇氢氧化钾在暗处、氮气与室温条件下制备茶类胡萝卜素提取率高、净度好、方法比较简单。制备的样品能满足高效液相色谱分析的要求。
High performance liquid chromatography (HPLC) was used to prepare two of carotenoidsfrom tea leaf. The results showed that carotenoids prepared using hexane-acetone-ethanol-toluene (10: 7: 6: 7) and 40% potassium hydroxide methanol solution at conditions of dark,nitrogen and room temperaturehad a good purity, The extraction yield was high and the method is simple and rnet the demand of HPLC analysis.
出处
《茶叶》
1998年第4期208-209,212,共3页
Journal of Tea
