摘要
[目的]建立适用于菜籽蛋白质组研究的双向电泳技术。[方法]以湘油17号为材料,对双向电泳技术中的样品制备方法、凝胶浓度、上样量等条件进行了优化。[结果]使用TCA-丙酮法提取菜籽总蛋白最佳,所获蛋白在2-DE图谱上获得的点数最多。当采用pH3～10IPG胶条和12%的凝胶、上样量为250μg时,可得到背景清晰、重复性好、蛋白点分辨率较高的双向电泳图谱。[结论]为开展油菜种子蛋白质组学研究奠定了基础。
[ Objective ] To establish the two-dimensional electrophoresis （2D-E） technology which was suitable for the research of rapeseed proteome. [ Method] Xiangyou 17 as the material, the sample preparation method, gel concentration and loading amount, etc. in 2D-E tech- nology were optimized. [ Result] The best extraction method of rapeseed total protein was TCA-acetone method, and the points on 2D-E map were the most. When IPG （pH 3 - 10） and 12% gel was used, and the loading amount was 250 μg, the two-dimensional electrophoresis map with the clear background, good repeatability and high protein point resolution was obtained. [ Conclusion ] The research laid the foundation for carrving out the rapeseed Droteomics research.
出处
《安徽农业科学》
CAS
北大核心
2011年第17期10178-10181,共4页
Journal of Anhui Agricultural Sciences
基金
国家863项目(2010AA101503)
国家科技支撑计划项目(2006BAD05A12)
合肥工业大学学生创新基金项目(XS2010100)
关键词
菜籽
蛋白质组
双向电泳
体系优化
Rapeseed
Proteome
Two-dimensional electrophoresis
System optimization
