摘要
本文报导了６种不同基因型甜菜叶柄的离体培养结果。实验通过预培养、诱导分化培养，获得了再生植株，建立了甜菜快速繁殖程序。结果表明：甜菜幼苗先经ＭＳＢ附加一定量的６－苄氨基嘌呤（６－ＢＡ）和萘乙酸（ＮＡＡ）组合的培养基中预培养，再取叶柄（带叶片）转入ＭＳ附加６－ＢＡ和ＮＡＡ的培养基中诱导分化，不定芽发生频率为１８．３％～４７．５％，不同基因型材料，诱导率不同。采用ＭＳＢ或ＭＳ大量元素减半，附加一定量的萘乙酸生根培养基，诱导生根率在８０％以上，移栽成活率高达１０％。
The results were reported with Sugarbeet petiole of six different genotypes in vitro.Plant regeneration was obtained .A rapid propagation system was establised,The result shows that shoot directly inducted from petiole in MS medium supplemented with Suitable amount of 6BA and NAA after preculture in MSB medium. Shoot induction was in high frequency which was 18.3%～47.5%,and different genotype, showed different induction frequency.Roots were induced on MS or MSB medium with a half their major elements supplement with suitable amount of NAA. The induced rooting about 80% and survial rate of the transplants was in high frequency reached 100%.
出处
《中国糖料》
1998年第4期21-24,共4页
Sugar Crops of China
关键词
甜菜
叶柄外植体
离体培养
植株再生
Sugarbeet Petiole explant In vitro Plant regeneration
