摘要
选用沿海甜菜(BetamaritimaL.)幼嫩花序顶端为试验材料,建立了一个快速高效的离体培养体系。在附加1mg/L6-BA的MS培养基上培养6周可诱导出丛生芽,诱导率达90%左右。丛生芽在适宜培养基上可快速扩增和长期继代培养。在附加1mg/LNAA的MS培养基上诱导小苗生根,生根率可达90%。再生植株经炼苗后移栽成活率为70%～80%。
A method for rapid and high efficient in vitro multiplication of immature inflorescence tips was established on Beta maritima L..The multiple bud clumps would be induced on MS medium supplemented with1mg/L6-benzylaminopurine (6-BA)for six weeks,the induction frequency would reach about 90%.The multiple bud clumps proliferated vigorously and could be subcultured for a long time on the proper MS medium.The seedlings were induced to root on MS medium supplemented with1mg/L naphthalene acetic acid(NAA),the rooting rate could reach90%.The maximum survival ratio of regeneration plants would be70%～80%after transplanted to pots.
出处
《中国糖料》
2003年第4期1-4,共4页
Sugar Crops of China
基金
国家863资助项目(2001AA627020)
关键词
沿海甜菜
花序
丛生芽
离体培养
Beta maritima L.
Inflorescence
Multiple bud clumps
In vitro culture
